Protease Inhibitor:Article Title: Neuronal-enriched small extracellular vesicles trigger a PD-L1-mediated broad suppression of T cells in Parkinson's disease.
Article Snippet: .. 40,6-diamidino-2-phenylindole (DAPI) Thermo Fisher Scientific Cat# D1306 protease inhibitor cocktail Sigma-Aldrich Cat# P2714 bovine serum albumin Sigma-Aldrich Cat# A1933-100G BMS-1166 Selleck Cat# S8859 lymphocyte separation medium CEDARLANE Cat# CL5030 Critical commercial assays MojoSort Mouse CD4 T cell Isolation Kit BioLegend Cat# 480006 MojoSort Mouse CD8 T cell Isolation Kit BioLegend Cat# 480008 PrimeScript RT reagent Kit with gDNA Eraser TaKaRa Cat# RR047A Dil-Membrane EVs Labeling & Purification Kit Rengen Biosciences Cat# EXOPDiI10-1 Experimental models: Cell lines Human iPSC lines carrying A53T mutant SNCA NINDS Human Cell and Data Repository Cat# ND50086; RRID: CVCL_RY37 Human iPSC lines carrying A53T mutation-corrected SNCA NINDS Human Cell and Data Repository Cat# ND50085; RRID: CVCL_RY36 SN4741 cell line Emory University School of Medicine RRID: CVCL_S466 HEK293T cell line American Type Culture Collection Cat# CRL-3216; RRID: CVCL_0063 .. C57/BL6J WT mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:000668 PITX3 +/IRES2 tTA transgenic mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:021962 tetO-A53T transgenic mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:012442
Cell Isolation:Article Title: Neuronal-enriched small extracellular vesicles trigger a PD-L1-mediated broad suppression of T cells in Parkinson's disease.
Article Snippet: .. 40,6-diamidino-2-phenylindole (DAPI) Thermo Fisher Scientific Cat# D1306 protease inhibitor cocktail Sigma-Aldrich Cat# P2714 bovine serum albumin Sigma-Aldrich Cat# A1933-100G BMS-1166 Selleck Cat# S8859 lymphocyte separation medium CEDARLANE Cat# CL5030 Critical commercial assays MojoSort Mouse CD4 T cell Isolation Kit BioLegend Cat# 480006 MojoSort Mouse CD8 T cell Isolation Kit BioLegend Cat# 480008 PrimeScript RT reagent Kit with gDNA Eraser TaKaRa Cat# RR047A Dil-Membrane EVs Labeling & Purification Kit Rengen Biosciences Cat# EXOPDiI10-1 Experimental models: Cell lines Human iPSC lines carrying A53T mutant SNCA NINDS Human Cell and Data Repository Cat# ND50086; RRID: CVCL_RY37 Human iPSC lines carrying A53T mutation-corrected SNCA NINDS Human Cell and Data Repository Cat# ND50085; RRID: CVCL_RY36 SN4741 cell line Emory University School of Medicine RRID: CVCL_S466 HEK293T cell line American Type Culture Collection Cat# CRL-3216; RRID: CVCL_0063 .. C57/BL6J WT mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:000668 PITX3 +/IRES2 tTA transgenic mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:021962 tetO-A53T transgenic mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:012442
Labeling:Article Title: Neuronal-enriched small extracellular vesicles trigger a PD-L1-mediated broad suppression of T cells in Parkinson's disease.
Article Snippet: .. 40,6-diamidino-2-phenylindole (DAPI) Thermo Fisher Scientific Cat# D1306 protease inhibitor cocktail Sigma-Aldrich Cat# P2714 bovine serum albumin Sigma-Aldrich Cat# A1933-100G BMS-1166 Selleck Cat# S8859 lymphocyte separation medium CEDARLANE Cat# CL5030 Critical commercial assays MojoSort Mouse CD4 T cell Isolation Kit BioLegend Cat# 480006 MojoSort Mouse CD8 T cell Isolation Kit BioLegend Cat# 480008 PrimeScript RT reagent Kit with gDNA Eraser TaKaRa Cat# RR047A Dil-Membrane EVs Labeling & Purification Kit Rengen Biosciences Cat# EXOPDiI10-1 Experimental models: Cell lines Human iPSC lines carrying A53T mutant SNCA NINDS Human Cell and Data Repository Cat# ND50086; RRID: CVCL_RY37 Human iPSC lines carrying A53T mutation-corrected SNCA NINDS Human Cell and Data Repository Cat# ND50085; RRID: CVCL_RY36 SN4741 cell line Emory University School of Medicine RRID: CVCL_S466 HEK293T cell line American Type Culture Collection Cat# CRL-3216; RRID: CVCL_0063 .. C57/BL6J WT mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:000668 PITX3 +/IRES2 tTA transgenic mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:021962 tetO-A53T transgenic mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:012442
Purification:Article Title: Neuronal-enriched small extracellular vesicles trigger a PD-L1-mediated broad suppression of T cells in Parkinson's disease.
Article Snippet: .. 40,6-diamidino-2-phenylindole (DAPI) Thermo Fisher Scientific Cat# D1306 protease inhibitor cocktail Sigma-Aldrich Cat# P2714 bovine serum albumin Sigma-Aldrich Cat# A1933-100G BMS-1166 Selleck Cat# S8859 lymphocyte separation medium CEDARLANE Cat# CL5030 Critical commercial assays MojoSort Mouse CD4 T cell Isolation Kit BioLegend Cat# 480006 MojoSort Mouse CD8 T cell Isolation Kit BioLegend Cat# 480008 PrimeScript RT reagent Kit with gDNA Eraser TaKaRa Cat# RR047A Dil-Membrane EVs Labeling & Purification Kit Rengen Biosciences Cat# EXOPDiI10-1 Experimental models: Cell lines Human iPSC lines carrying A53T mutant SNCA NINDS Human Cell and Data Repository Cat# ND50086; RRID: CVCL_RY37 Human iPSC lines carrying A53T mutation-corrected SNCA NINDS Human Cell and Data Repository Cat# ND50085; RRID: CVCL_RY36 SN4741 cell line Emory University School of Medicine RRID: CVCL_S466 HEK293T cell line American Type Culture Collection Cat# CRL-3216; RRID: CVCL_0063 .. C57/BL6J WT mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:000668 PITX3 +/IRES2 tTA transgenic mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:021962 tetO-A53T transgenic mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:012442
Mutagenesis:Article Title: Neuronal-enriched small extracellular vesicles trigger a PD-L1-mediated broad suppression of T cells in Parkinson's disease.
Article Snippet: .. 40,6-diamidino-2-phenylindole (DAPI) Thermo Fisher Scientific Cat# D1306 protease inhibitor cocktail Sigma-Aldrich Cat# P2714 bovine serum albumin Sigma-Aldrich Cat# A1933-100G BMS-1166 Selleck Cat# S8859 lymphocyte separation medium CEDARLANE Cat# CL5030 Critical commercial assays MojoSort Mouse CD4 T cell Isolation Kit BioLegend Cat# 480006 MojoSort Mouse CD8 T cell Isolation Kit BioLegend Cat# 480008 PrimeScript RT reagent Kit with gDNA Eraser TaKaRa Cat# RR047A Dil-Membrane EVs Labeling & Purification Kit Rengen Biosciences Cat# EXOPDiI10-1 Experimental models: Cell lines Human iPSC lines carrying A53T mutant SNCA NINDS Human Cell and Data Repository Cat# ND50086; RRID: CVCL_RY37 Human iPSC lines carrying A53T mutation-corrected SNCA NINDS Human Cell and Data Repository Cat# ND50085; RRID: CVCL_RY36 SN4741 cell line Emory University School of Medicine RRID: CVCL_S466 HEK293T cell line American Type Culture Collection Cat# CRL-3216; RRID: CVCL_0063 .. C57/BL6J WT mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:000668 PITX3 +/IRES2 tTA transgenic mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:021962 tetO-A53T transgenic mice Jackson Laboratory, Bar Harbor, ME RRID: IMSR_JAX:012442
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